The Present invention provide technique for improvements as solution to hypoxia generated in conventional plant tissue culture practices. It Provides a chemical compositions when used in effective concentration will improve plants yield, hypoxic tolerance of plants micro propagated through plant tissue culture practice. And, Chemical composition comprises high concentration of potassium nitrate, choline chloride, glutamine acid, L- Glycine, L-Cysteine, Adenine sulphate, D Ca- Pantothenate acid, Sodium silicate and succinic acid derivatives along with plant tissue culture medium and plant growth hormones. Said chemical compositions may be added during multiplication stage, shooting stage and rooting stage along with plant tissue culture medium.
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