Invention relates to a method of identifying small molecule inhibitors for the HIV TAT using a high throughput screening (HTS) assay, which will aid in the development of new class of HIV drugs. It relates to a TAT-inducible GFP anti RFP shRNA vector, vector combinations, recombinant cell having vectors comprising nucleotide sequence sets. The technology can effectively distinguish between a genuine anti-viral inhibitor and a cytotoxic molecule and thus , decreases the number of false hits.
For more details about technology:: Click here